Diffraction Techniques

Diffraction techniques core facility provides services for characterization of protein structure using single crystal X-ray diffraction and small-angle X ray scattering for analysis of liquid samples. The quality of high-intense X-ray beams is enabled by using of MetalJet sources (Excillum). The service provided includes data processing and reporting ready for publication.

Key Equipment

D8 Venture diffractometer (Bruker)
- Single crystal diffractometer with a high-flux liquid Gallium X-ray source MetalJet D2, Photon II detector and Kappa goniometer
- High resolution studies in cryogenic conditions
- ISX stage (Bruker) - motorized stage for in-situ X-ray diffraction experiments, enabling screening of diffraction properties in crystallization plates
- HC-Lab - controlled humidity of crystal environment for crystal dehydratation and room temperature experiments
SAXSpoint 2.0 (Anton Paar)
- Measurement of SAXS or WAXS
- The instrument is equipped with MetalJet C2+ X-ray source (Excillum), Eiger 1M detector (Dectris)
- Automatic sample delivery
- In-situ UV-Vis spectrometer (Agilent)
- Liquid chromatography system (Akta Go, GE Healthcare) for SEC-SAXS experiments

Expertise

Screening for X-ray diffraction of macromolecules
In-house data collection and processing
In-situ evaluation of X-ray diffraction of sensitive crystallization targets
Experimental phasing experiments – evaluation for MIR, SAD and MAD approaches
SAXS data measurement and evaluation, including SEC-SAXS
In-situ UV-Vis monitoring
Synchrotron-based data collection and processing, structure solution

Open-access

Assisted use of all experimental equipment, hosting prolonged experiments (in-house experimental phasing), “service” data collection at synchrotron sources of radiation in individual cases, capacity dedicated to methods development, long-term documented cryo-storage