Crystallization of Proteins and Nucleic Acids
Crystallization of Proteins and Nucleic Acids core facility provides services focused on obtaining crystals of biomacromolecules and their complexes. We support both manual and robotic crystallization drops setting under various conditions. Remote control of crystal growth in the crystallization hotel is provided using the web interface over Internet.
Key Equipment
- Gryphon dropsetter (ArtRobins) - a multi-channel (96 channels) pipetting robot for the easy set-up of nanodrop crystallization plates
- Crystallization hotel RI1000 (Formulatrix) - a crystallization plate storage and automated crystallization monitoring enclosure allowing remote access to crystallization images
- Glovebox with stereomicroscope (GS) for crystallization and crystal manipulation under defined (oxygen-free) atmosphere
- Dropsetter NT8 (Formulatrix) – Automated pipetting crystallisation robot with controlled level of the air humidity, LCP head, possibility of robotic seeding
- Crystallization hotel RI182 (Formulatrix) - allows storing crystallization plates at 10 °C and image them according to the pre-set time schedule in visible light, polarized light, and UV light
- SpectroLight 600 (Xtal Concepts)
- SONICC (Formulatrix)
- Vitrobot (Thermo Scientific)
Expertise
- Classical and robotic crystallogenesis with remote experiment monitoring in three dedicated laboratories with strictly controlled temperature regimes with full backup
- Automated screening of variety of crystallization conditions and its effects
- Anaerobic crystallization and manipulation of biomacromolecules
Open-access
- Assisted use of all experimental equipment for crystallogenesis, limited capacity for service crystallogenesis
Core Facility contact
- Jiří Pavlíček - jiri.pavlicek@ibt.cas.cz
- Jan Stránský - Jan.Stransky@ibt.cas.cz
Do you want to use this equipment or services?
More information about Josef Dadok National NMR Centre and related services you can find in document Equipement and services of CIISB (page 17).
