Structural mass spectrometry

BIOCEV Centre of molecular structure provides access to technologies installed in the newly constructed building of the Centre in Vestec near Prague.

Key Equipment

  • Bruker Daltonics 15T-Solarix XR FT-ICR mass spectrometer, with electrospray and MALDI ion sources
  • Agilent Technologies 1200 HPLC system (usually coupled to the 15T-SolariX XR mass spectrometer) for the separation of complex peptide mixtures, proteins and metabolites

Expertise

  • The utilization of high resolution mass spectrometry (15T FT-ICR MS) to determine the composition of molecules (metabolites, nucleic acid, proteins, and carbohydrates) based on accurate mass measurements and fragment pattern. FT-ICR MS will be equipped with atmospheric pressure ionization technique (electrospray) and various types of vacuum ionization techniques (laser desorption, matrix-assisted laser desorption)
  • FT-ICR MS will be able to perform sustained off-resonance irradiation, collision-induced and electron transfer/capture dissociations
  • Mass spectrometric cutting-edge analysis of post-translational modifications, and of structural states of proteins and complexes in solution
  • Combination of covalent surface labelling, isotope (H/D) exchange, chemical cross-linking

Open-access

  • Separation of protein mixtures
  • Intact protein analysis
  • Peptide/protein/metabolite profiling
  • Protein identification
  • Characterization of protein modifications
  • Protein/metabolite quantification
  • Protein surface covalent labelling
  • Chemical cross-linking
  • H/D exchange
  • Data processing and interpretation of mass spectrometric data

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More information about Structural mass spectrometry and related services you can find in document Equipement and services of CIISB (page 17).


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