Diffraction techniques

BIOCEV Centre of molecular structure provides access to technologies installed in the newly constructed building of the Centre in Vestec near Prague.

Key Equipment

    • Bruker D8 Venture diffractometer with a high-flux liquid Gallium X-ray source MetalJet D2, Photon II detector and Kappa goniometer 
    • ISX stage for D8 Venture – motorized stage for in-situ X-ray diffraction experiments, enabling screening of diffraction properties in crystallization trays

Expertise

    • Screening for X-ray diffraction of macromolecules
    • In-house data collection and processing,
    • In-situ evaluation of sensitive crystallisation targets, experimental phasing experiments – evaluation for MIR, SAD and MAD approaches
    • Synchrotron-based data collection and processing, structure

Open-access

    • Assisted use of all experimental equipment, hosting prolonged experiments (in-house experimental phasing), “service” data collection at synchrotron sources of radiation in individual cases, capacity dedicated to methods development, long-term documented cryo-storage

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