Proteomics

Mass spectrometry – based proteomics. Activities of the Core Facility cover all steps of proteomic analysis – protein isolation, separation of protein mixtures, protein characterisation by mass spectrometry and bioinformatic data processing.

Key Equipment

    • High-resolution mass spectrometer (FTMS) with Orbitrap
    • Hybrid mass spectrometer (Q-LIT) with quadrupole and linear ion trap and liquid chromatography system
    • MALDI-TOF/TOF mass spectrometer

Expertise

  • All steps of proteomic analysis - protein isolation, separation of protein mixtures, protein characterization by mass spectrometry and bioinformatics data processing.

Open-access

    • Measurement of NMR spectra at magnetic fields ranging from 11.75 T to 22.32 T (corresponding to proton frequencies 500 MHz to 950 MHz)
    • Separation of protein mixtures
    • Intact protein analysis
    • Peptide/protein profiling (e.g. microorganism characterization)
    • Protein identification
    • Characterization of protein modifications
    • Protein quantification

Core Facility contact

Zbyněk Zdráhal - zbynek.zdrahal@ceitec.muni.cz

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More information about Josef Dadok National NMR Centre and related services you can find in document Equipement and services of CIISB (page 17).


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