Czech Infrastructure for Integrative Structural Biology – CIISB
A gateway to realm of structural data for biochemists, biophysicists, molecular biologist, and all scientists whose research benefits from accurate structure determination of biological macromolecules, assemblies, and complex molecular machineries at atomic resolution.
Open access to 10 high-end core facilities and assisted expertise in NMR, X-ray crystallography and crystallization, cryo-electron microscopy and tomography, biophysical characterization of biomolecular interaction, nanobiotechnology, proteomics and structural mass spectrometry.
Czech national centre of European Research Infrastructure Consortium INSTRUCT ERIC.
CEITEC Core Facilities
BIOCEV Core Facilities
Professor Armin Delong passed away on October 5, 2017
a founder of electron microscopy in the former Czechoslovakia and prominent physicist, passed away on October 5, 2017 at the age of 92 (*29.1.1925 - ✝5.10.2017).
5th Call for proposals for the Instruct Internship programme
Instruct provides access to state-of-the-art infrastructure and technology to support research in structural biology.
2017 Nobel Prize in Chemistry - Cryo-electron Microscopy
The Nobel Prize in Chemistry 2017 was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution".
Monday – Thursday
23 Oct – 26 Oct
Second 4-day crystallization workshop will be focused on Biomacromolecular crystallization techniques.
Tuesday – Wednesday
24 Oct – 25 Oct
Study of functions and structures of human protein complexes and essential interactions of biomolecules in live cells.
Training in utilization of MALDI-MS profiling method for bacteria identification (focused on master and PhD students).
RNA polymerase II (RNAPII) not only transcribes protein coding genes and many noncoding RNA, but also coordinates transcription and RNA processing. This coordination is mediated by a long C-terminal domain (CTD) of the largest RNAPII subunit, which serves as a binding platform for many RNA/protein-binding factors involved in transcription regulation. In this work, we used a hybrid approach to visualize the architecture of the full-length CTD in complex with the transcription termination factor Rtt103. Specifically, we first solved the structures of the isolated subcomplexes at high resolution and then arranged them into the overall envelopes determined at low resolution by small-angle X-ray scattering. The reconstructed overall architecture of the Rtt103–CTD complex reveals how Rtt103 decorates the CTD platform.
Jasnovidova, O.; Klumpler, T.; Kubicek, K.; Kalynych, S.; Plevka, P.; Stefl, R. PNAS, Structure and dynamics of the RNAPII CTDsome with Rtt103 PNAS, published ahead of print October 4, 2017, doi:10.1073/pnas.1712450114
The first atomic resolution structure of a stable G-hairpin formed by a natively occurring DNA sequence is reported. An 11-nt long G-rich DNA oligonucleotide, 5′-d(GTGTGGGTGTG)-3′, corresponding to the most abundant sequence motif in irregular telomeric DNA from Saccharomyces cerevisiae adopts a novel type of mixed parallel/antiparallel fold-back DNA structure, which is stabilized by dynamic G:G base pairs that transit between N1-carbonyl symmetric and N1-carbonyl, N7-amino base-pairing arrangements. The structure reveals previously unknown principles of the folding of G-rich oligonucleotides that could be applied to the prediction of natural and/or the design of artificial recognition DNA elements. The structure also demonstrates that the folding landscapes of short DNA single strands is much more complex than previously assumed.
Gajarsky, M.; Zivkovic, M. L. ; Stadlbauer, P. ; Pagano, B.; Fiala, R.; Amato, J.; Tomaska, L.; Sponer, J.; Plavec, J.; Trantirek, L., Structure of a Stable G-Hairpin JACS 139, 3591-3594, doi:10.1021/jacs.6b10786 (2017)