Czech Infrastructure for Integrative Structural Biology – CIISB
A gateway to realm of structural data for biochemists, biophysicists, molecular biologist, and all scientists whose research benefits from accurate structure determination of biological macromolecules, assemblies, and complex molecular machineries at atomic resolution.
Open access to 10 high-end core facilities and assisted expertise in NMR, X-ray crystallography and crystallization, cryo-electron microscopy and tomography, biophysical characterization of biomolecular interaction, nanobiotechnology, proteomics and structural mass spectrometry.
A distributed infrastructure constituted by Core Facilities of CEITEC (Central European Institute of Technology), located in Brno, and BIOCEV (Biotechnology and Biomedicine Centre), located in Vestec near Prague, Central Bohemia.
Czech national centre of European Research Infrastructure Consortium INSTRUCT ERIC.
CEITEC Core Facilities
BIOCEV Core Facilities
Velké výzkumné infrastruktury - Operated by CESNET on behalf of the Council for Large Infrastructures for Research, Experimental Development and Innovation.
Nadrian C. Seeman: DNA, not Merely the Secret of Life
We build branched DNA species that can be joined using Watson-Crick base pairing to produce N-connected objects and lattices. We have used ligation to construct DNA topological targets, such as knots, polyhedral catenanes, Borromean rings and a Solomon's knot.
An Instruct/CIISB course took place at the CMS, IBT, Biocev on April 5 and 6, 2018.
This course/workshop allowed participants, with some prior knowledge and experience in macromolecular crystallography, to access the biophysical measurements section and the crystal handling/X-ray diffraction section of the Instruct CZ / CIISB CMS core facility for three practical work sessions.
XV. Discussions in Structural Molecular Biology and the 2nd User Meeting of the Czech Infrastructure for Integrative Structural Biology
In March, 2018, the Czech and Slovak community of structural biologists met at the "XV. Discussions in Structural Molecular Biology".
Wednesday – Friday
23 May – 25 May
The main topic of the 3-days workshop will be characterisation of sample - from thermal stability to its oligomeric state.Kamenice 753/5
625 00 Brno
Data acquisition with BioSAXS-1000 , ATSAS, ab initio and rigid body modeling in SAXS data analysis
Monday – Friday
3 Sep – 7 Sep
One week-course will be dedicated to various theoretical and practical aspects of the interactions on a molecular level.
Nat. Commun. 2018
Chemistry – A European Journal 2018
Tick-borne encephalitis virus (TBEV) causes 13,000 cases of human meningitis and encephalitis annually. However, the structure of the TBEV virion and its interactions with antibodies are unknown. Here, Pavel Plevka and his coworkers present cryo-EM structures of the native TBEV virion and its complex with Fab fragments of neutralizing antibody 19/1786. Flavivirus genome delivery depends on membrane fusion that is triggered at low pH. The virion structure indicates that the repulsive interactions of histidine side chains, which become protonated at low pH, may contribute to the disruption of heterotetramers of the TBEV envelope and membrane proteins and induce detachment of the envelope protein ectodomains from the virus membrane. The Fab fragments bind to 120 out of the 180 envelope glycoproteins of the TBEV virion. Unlike most of the previously studied flavivirus-neutralizing antibodies, the Fab fragments do not lock the E-proteins in the native-like arrangement, but interfere with the process of virus-induced membrane fusion.
Fuzik, T. et al. Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody. Nature Communications 9, 11, doi:10.1038/s41467-018-02882-0 (2018). doi:10.1038/s41467-018-02882-0
Photorhabdus asymbiotica is a gram‐negative bacterium that is not only as effective an insect pathogen as other members of the genus, but it also causes serious diseases in humans. The recently identified lectin PHL from P. asymbiotica verifiably modulates an immune response of humans and insects, which supports the idea that the lectin might play an important role in the host–pathogen interaction. Dimeric PHL contains up to seven l‐fucose‐specific binding sites per monomer, and in order to target multiple binding sites of PHL, α‐l‐fucoside‐containing di‐, tri‐ and tetravalent glycoclusters were synthesized. The interaction between fucoside derivates and PHL was investigated by several biophysical and biological methods, ITC and SPR measurements, hemagglutination inhibition assay, and an investigation of bacterial aggregation properties were carried out. Details of the interaction between PHL and propargyl α‐l‐fucoside as a monomer unit were revealed using X‐ray crystallography. Besides this, the interaction with multivalent compounds was studied by NMR techniques. The newly synthesized multivalent fucoclusters proved to be up to several orders of magnitude better ligands than the natural ligand, l‐fucose.
Jancarikova, G. et al. Synthesis of a-l-Fucopyranoside-Presenting Glycoclusters and Investigation of Their Interaction with Photorhabdus asymbiotica Lectin (PHL). Chemistry-A European Journal, 24, 4055-4068, doi.org/10.1002/chem.201705853