New services in Protein production core facility

We are pleased to announce the expansion of our Protein Production Core Facility with the introduction of eukaryotic protein production. In addition to prokaryotic expression in E. coli, we now offer protein production in human embryonic kidney cells (HEK) and Sf9 insect cells.

Baculoviral Expression in Insect Cells
For high-yield protein production in Sf9 and High Five™ insect cells, we use two baculoviral expression systems: BacPAK™ (TaKaRa) and FlexiBAC. These systems allow for the efficient and scalable production of recombinant proteins.

• Small-scale transfection of insect cells to assess protein expression.
• Verification of expression using Western blot analysis.
• Amplification of baculovirus stocks to generate high-titer viral particles.
• Large-scale protein production and purification of the recombinant protein.

Mammalian Expression Systems

For mammalian protein expression, we use HEK293T/17SF suspension cells, Expi293™ cells for high density cultures and enhanced protein yield, and Expi293F™ GnTI- cells. This strain ensures the expression of homogenously glycosylated proteins and lacks complex N-glycans.

• Small-scale transfection of mammalian cells for initial expression screening.
• Verification of expression using Western blot analysis.
• Optimization and scale-up for large-scale production.
• Purification of recombinant proteins using standard and custom purification protocols.

Whether you require a complete workflow from gene cloning to purified protein or need assistance with a specific step, we can tailor our services to meet your project’s requirements. Please contact us to discuss how we can support your research.