A fluorescence light microscope for imaging of fluorescently labeled samples at cryo-conditions has been installed at Cryo-electron microscopy core facility CEITEC MU (CEMCOF). The purchase of the instrument is a part of the facility upgrade and further development financed by UP CIISB project. The setup is composed of a Leica DM6 FS microscope equipped with the cryo-stage and dedicated 50x cryo-objective with NA of 0.9. The system is further equipped with THUNDER technology for real-time deconvolution of the data during acquisition. The microscope will become an indispensable part of the correlative light and electron microscopy imaging (CLEM) of biological specimens at the near-native conditions. Following the proof-of-concept studies, the cryo-CLEM workflow comprising sample vitrification (plunge freezing of high-pressure freezing), cryo-FIB lamella preparation, cryo-fluorescence microscopy, cryo-electron tomography will be included among the services provided at CEMCOF.
This is a reminder to apply for the Instruct-ERIC R&D Pilot Call - get funding of up to €15,000!
Closing 14:00 CEST on 15 April, the Instruct-ERIC R&D Funding Call invites early career researchers to submit proposals to access Instruct technology, equipment, and expertise from our 27 facilities across Europe for small scale pilot research projects in integrated structural biology.
Register now for the 32nd edition of the Instruct-ERIC Structure Meets Function webinar series, taking place on Zoom, 17 April at 11:00 CEST.
The latest webinar will have two speakers who have accessed the Instruct-ERIC facilities, expertise, and machinery via the ISIDORe project.
Talk 1: Nikitas Georgiou - National and Kapodistrian University of Athens
Title: Potential anti-inflammatory drugs
Talk 2: Séverine Padiolleau - Université de Technologie de Compiègne
Title: Identification of aptamers interacting with a protein target involved in lyme disease