Widefield cryo-light microscope for correlative light and electron microscopy imaging

1 Sep 2021

A fluorescence light microscope for imaging of fluorescently labeled samples at cryo-conditions has been installed at Cryo-electron microscopy core facility CEITEC MU (CEMCOF). The purchase of the instrument is a part of the facility upgrade and further development financed by UP CIISB project. The setup is composed of a Leica DM6 FS microscope equipped with the cryo-stage and dedicated 50x cryo-objective with NA of 0.9. The system is further equipped with THUNDER technology for real-time deconvolution of the data during acquisition. The microscope will become an indispensable part of the correlative light and electron microscopy imaging (CLEM) of biological specimens at the near-native conditions. Following the proof-of-concept studies, the cryo-CLEM workflow comprising sample vitrification (plunge freezing of high-pressure freezing), cryo-FIB lamella preparation, cryo-fluorescence microscopy, cryo-electron tomography will be included among the services provided at CEMCOF.

No description


More articles

All articles

You are running an old browser version. We recommend updating your browser to its latest version.