Upgrades of the differential scanning fluorimetry instruments Prometheus NT.48 at BIOCEV and CEITEC
Recently, both instruments Prometheus NT.48 at BIOCEV and CEITEC, permitting precise measurements of the intrinsic fluorescence of proteins, thereby characterizing their thermal unfolding, have been upgraded to include back-reflection optics, which provides insight into the aggregation processes. The basis for such detection is the aggregation-induced light scattering by particles resulting in loss of light. The combination of thermal stability detection and determination of aggregation onset temperatures allows for the most rapid, precise, and information rich analysis in formulation screenings or protein engineering projects. The upgraded instruments now can provide comprehensive results for any type of proteins, and are especially suitable for the prediction of proteins stability, for the screening of buffer influence, and for the testing of storage conditions. Part of the upgrade was also increase in the maximum possible temperature from original 95°C to 110°C . This enhancement allows to characterize proteins with higher melting temperature. Both upgrades were financed from the European Regional Development Fund-Project „CIISB4HEALTH“ (No. CZ.02.1.01/0.0/0.0/16_013/0001776).
4th National Day of Large Research Infrastructures
The Ministry of Education, Youth and Sports of the Czech Republic and the Faculty of Mathematics and Physics of Charles University are honoured to invite you to take part in the 4th National Day of Large Research Infrastructures, to be held on 19th November 2019 in the premises of LINDAT/CLARIAH-CZlarge research infrastructure.
Instruct-ERIC International Access Call
We are delighted to announce the opening of the Instruct access call for International users.
Prof Wolfgang Baumeister awarded the 2019 Ivano Bertini Award for outstanding contributions to structural biology
The Ivano Bertini Award is offered by Instruct to recognise a significant achievement in frontier research that utilises an integrative structural biology approach.
CFs succeeded in getting EU support to organize a workshop on the sample quality assessment