Upgrade of the workflow for cryo-electron tomography and microscopy

8 Jul 2020

The CIISB Cryo-electron microscopy core facility at CEITEC Masaryk University is expanding its services in the sample preparation for electron microscopy. The facility has recently acquired high-pressure freezer Leica EM ICE for vitrification of bulky biological specimen (up to 200 mm thickness). In addition, the freeze-substitution unit Leica EM AFS2 for resin embedding of the high-pressure frozen samples and the ultramicrotom Leica EM UC7 with the adapter for cryo-ultramicrotomy were purchased in order to provide the facility users with the complete workflow for preparation of thin section samples for both room-temperature electron microscopy and cryo-electron microscopy.

The purchase of the instrument was financed by MEYS CR from the Operational Programme: Research, Development and Education, the project „CIISB UP“ (No. CZ.02.1.01/0.0/0.0/18_046/0015974).

16 Apr

Open position Junior/medior backend developer in data management (IBT Biocev)

The Institute of Biotechnology of the Academy of Sciences of the Czech Republic at the Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec announces an open position:

Junior/medior backend developer in data management

We are looking for an enthusiast to join our team focused on management of biophysical data within the European project MOSBRI and the National Repository Platform.

We offer work connected to development of data management infrastructure for biophysical data in the frame of an international project at the Institute of Biotechnology in the BIOCEV Centre of Excellence.
1 Apr

New services in Protein production core facility

We are pleased to announce the expansion of our Protein Production Core Facility with the introduction of eukaryotic protein production. In addition to prokaryotic expression in E. coli, we now offer protein production in human embryonic kidney cells (HEK) and Sf9 insect cells.
31 Mar

New instrumentation in Proteomics core facility

In March, two new high resolution mass spectrometers were installed in our core facility thanks to CIISB project (CZ.02.01.01/00/23_015/0008175). First we upgraded our timsTOF Pro 2 instrument to timsTOF HT improving our performance in whole proteome analyses with faster scanning speed and a wider dynamic range. In second, Ultra 2 mass spectrometer was installed. This timsTOF instrument opens doors to deeper analysis of samples with limited amount of input material due to its ultimate sensitivity.
7 Mar

Launch of database for raw molecular biophysics data - MBDB

We have launched a public database where you can store your raw data with rich annotation of samples and conditions of measurements.

The Molecular Biophysics Database currently accepts depositions of raw measurement files from MST, BLI, SPR and ITC.

We invite you to deposit your data, make it findable, searchable and reusable at https://mbdb-data.org/

MBDB has been developed within the EU project MOSBRI by a team of developers mainly from the Institute of Biotechnology and CESNET. Its development continues and we welcome comments and suggestions at mbdb@ibt.cas.cz.

All articles

Upgrade of the workflow for cryo-electron tomography and microscopy

More articles

Open position Junior/medior backend developer in data management (IBT Biocev)

New services in Protein production core facility

New instrumentation in Proteomics core facility

Launch of database for raw molecular biophysics data - MBDB