Transmission electron microscope Titan Krios at Cryo-electron microscopy and tomography core facility CEITEC MU has been recently upgraded with phase plate, and two last generation direct electron detectors (FEI Falcon3EC, Gatan K2). Phase plate significantly increases contrast in cryo-electron microscopy data and allows to collect data at in-focus conditions. Moreover, increased contrast extends applicability of cryo-electron microscopy to high-resolution structural characterization of proteins and protein complexes which are around 100kDa in size. Both newly acquired direct electron detectors operate in electron counting mode, which provides superior image quality. Besides, the Gatan K2 detector position is behind the post-column energy filter and allows zero-loss imaging. Such application is primarily beneficial for imaging of thicker samples such as vitrified cells or focused ion beam micromachined cellular lamellas using cryo-electron tomography for the purpose of structural characterization of biomacromolecular complexes in situ. All upgrades were financed from the European Regional Development Fund-Project „CIISB4HEALTH“ (No. CZ.02.1.01/0.0/0.0/16_013/0001776).
This is a reminder to apply for the Instruct-ERIC R&D Pilot Call - get funding of up to €15,000!
Closing 14:00 CEST on 15 April, the Instruct-ERIC R&D Funding Call invites early career researchers to submit proposals to access Instruct technology, equipment, and expertise from our 27 facilities across Europe for small scale pilot research projects in integrated structural biology.
Register now for the 32nd edition of the Instruct-ERIC Structure Meets Function webinar series, taking place on Zoom, 17 April at 11:00 CEST.
The latest webinar will have two speakers who have accessed the Instruct-ERIC facilities, expertise, and machinery via the ISIDORe project.
Talk 1: Nikitas Georgiou - National and Kapodistrian University of Athens
Title: Potential anti-inflammatory drugs
Talk 2: Séverine Padiolleau - Université de Technologie de Compiègne
Title: Identification of aptamers interacting with a protein target involved in lyme disease