Titan Krios transmission electron microscope at CEITEC equipped with phase plate and last generation direct electron detectors
Transmission electron microscope Titan Krios at Cryo-electron microscopy and tomography core facility CEITEC MU has been recently upgraded with phase plate, and two last generation direct electron detectors (FEI Falcon3EC, Gatan K2). Phase plate significantly increases contrast in cryo-electron microscopy data and allows to collect data at in-focus conditions. Moreover, increased contrast extends applicability of cryo-electron microscopy to high-resolution structural characterization of proteins and protein complexes which are around 100kDa in size. Both newly acquired direct electron detectors operate in electron counting mode, which provides superior image quality. Besides, the Gatan K2 detector position is behind the post-column energy filter and allows zero-loss imaging. Such application is primarily beneficial for imaging of thicker samples such as vitrified cells or focused ion beam micromachined cellular lamellas using cryo-electron tomography for the purpose of structural characterization of biomacromolecular complexes in situ. All upgrades were financed from the European Regional Development Fund-Project „CIISB4HEALTH“ (No. CZ.02.1.01/0.0/0.0/16_013/0001776).
UP CIISB Kick-Off Meeting, Monday, December 2, 2019
UP CIISB Kick-Off Meeting will gather the CIISB Core Facility Heads, Executive Committee members, and administration staff of CEITEC and BIOCEV to discuss the starting OP VVV investment project UP CIISB.
Petr Sedmera Prize 2020 - Call for Nominations Announced
The Petr Sedmera Prize is awarded for best published work in the field of nuclear magnetic resonance and is dedicated to the memory of a leading expert who has been instrumental in the development of NMR in Czechoslovakia and the Czech Republic.
What’s ahead of us - A growing number of research agencies is assigning money randomly
Using lottery as a fairer and more open system in awarding grants? Read an interesting Nature’s article about a new trend.
Update of roadmap of Large Research Infrastructures of the Czech Republic
A new update of Roadmap of Large Research Infrastructures of the Czech Republic is now available.