Titan Krios transmission electron microscope at CEITEC equipped with phase plate and last generation direct electron detectors
Transmission electron microscope Titan Krios at Cryo-electron microscopy and tomography core facility CEITEC MU has been recently upgraded with phase plate, and two last generation direct electron detectors (FEI Falcon3EC, Gatan K2). Phase plate significantly increases contrast in cryo-electron microscopy data and allows to collect data at in-focus conditions. Moreover, increased contrast extends applicability of cryo-electron microscopy to high-resolution structural characterization of proteins and protein complexes which are around 100kDa in size. Both newly acquired direct electron detectors operate in electron counting mode, which provides superior image quality. Besides, the Gatan K2 detector position is behind the post-column energy filter and allows zero-loss imaging. Such application is primarily beneficial for imaging of thicker samples such as vitrified cells or focused ion beam micromachined cellular lamellas using cryo-electron tomography for the purpose of structural characterization of biomacromolecular complexes in situ. All upgrades were financed from the European Regional Development Fund-Project „CIISB4HEALTH“ (No. CZ.02.1.01/0.0/0.0/16_013/0001776).
Instruct-ERIC workshop on Computational Approaches in Integration of Structural Biology Techniques
On October 8 – 10, the Institute of Biotechnology of the Czech Academy of Sciences organised an Instruct – ERIC workshop on Computational Approaches in Integration of Structural Biology Techniques.
The 2019 Nobel Prize in Chemistry
Is awarded to John Goodenough, M. Stanley Whittingham and Akira Yoshino “for the development of lithium-ion batteries”.
The Nobel Prize in Physiology or Medicine 2019
is awarded jointly to William G. Kaelin Jr, Sir Peter J. Ratcliffe and Gregg L. Semenza “for their discoveries of how cells sense and adapt to oxygen availability.”
The Nobel Prize in Physics 2019
is awarded ”for contributions to our understanding of the evolution of the universe and Earth’s place in the cosmos”, with one half to James Peebles “for theoretical discoveries in physical cosmology” and the other half jointly to Michel Mayor and Didier Queloz “for the discovery of an exoplanet orbiting a solar-type star.”