Plasma-FIB/SEM microscope for life-science applications installed at CEITEC

6 May 2023

Helios Hydra V (ThermoScientific) comprises immersion SEM column for high-resolution SEM imaging and a four gas (Xe,Ar,O,N) plasma-FIB for efficient material ablation.
The available services will focus on the targeted lamella preparation using correlative light-electron microscopy and other correlative studies thanks to the wide-field fluorescence microscope integrated to the SEM chamber. In addition, Helios Hydra is available for FIB/SEM volume imaging under cryo- and room-temperatures conditions to support research focusing on ultrastructural analysis of the whole cells and tissue regions.

Correlative cryo-SEM and cryo-FM imaging of the pancreatic INS-1E cells (right). The cellular membrane (red) and mitochodria (green) were labeled dyes for live cell imaging. The data were collected using Helios Hydra V (left) equipped with the integrated flourescence microscope.

16 Apr

Open position Junior/medior backend developer in data management (IBT Biocev)

The Institute of Biotechnology of the Academy of Sciences of the Czech Republic at the Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec announces an open position:

Junior/medior backend developer in data management

We are looking for an enthusiast to join our team focused on management of biophysical data within the European project MOSBRI and the National Repository Platform.

We offer work connected to development of data management infrastructure for biophysical data in the frame of an international project at the Institute of Biotechnology in the BIOCEV Centre of Excellence.
1 Apr

New services in Protein production core facility

We are pleased to announce the expansion of our Protein Production Core Facility with the introduction of eukaryotic protein production. In addition to prokaryotic expression in E. coli, we now offer protein production in human embryonic kidney cells (HEK) and Sf9 insect cells.
31 Mar

New instrumentation in Proteomics core facility

In March, two new high resolution mass spectrometers were installed in our core facility thanks to CIISB project (CZ.02.01.01/00/23_015/0008175). First we upgraded our timsTOF Pro 2 instrument to timsTOF HT improving our performance in whole proteome analyses with faster scanning speed and a wider dynamic range. In second, Ultra 2 mass spectrometer was installed. This timsTOF instrument opens doors to deeper analysis of samples with limited amount of input material due to its ultimate sensitivity.
7 Mar

Launch of database for raw molecular biophysics data - MBDB

We have launched a public database where you can store your raw data with rich annotation of samples and conditions of measurements.

The Molecular Biophysics Database currently accepts depositions of raw measurement files from MST, BLI, SPR and ITC.

We invite you to deposit your data, make it findable, searchable and reusable at https://mbdb-data.org/

MBDB has been developed within the EU project MOSBRI by a team of developers mainly from the Institute of Biotechnology and CESNET. Its development continues and we welcome comments and suggestions at mbdb@ibt.cas.cz.

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Plasma-FIB/SEM microscope for life-science applications installed at CEITEC

More articles

Open position Junior/medior backend developer in data management (IBT Biocev)

New services in Protein production core facility

New instrumentation in Proteomics core facility

Launch of database for raw molecular biophysics data - MBDB