PDB-Dev: A Prototype System for Depositing Integrative/Hybrid Structural Models
Essential mechanisms in biology frequently involve large macromolecular assemblies (or machines). In favorable cases, their structures can be determined by X-ray crystallography or nuclear magnetic resonance (NMR) spectroscopy or electron microscopy (3DEM) alone, culminating in deposition of atomic structural models (with x, y, z atomic coordinates) into the global PDB archive (PDB; pdb.org). Many such biological machines are, however, poorly suited to single experimental method approaches. Researchers are increasingly forced to combine various experimental data and information from measurements and computational analyses to generate “hybrid” or “integrative” structural models of macromolecular assemblies. PDB-Dev is a prototype archiving system for structural models obtained through integrative/hybrid (I/H) methods. Structural characterization of many complex macromolecular assemblies is increasingly carried out using I/H methods, where a combination of complementary experimental and computational techniques is used to determine the structure. The structural models obtained through I/H methods are collected, archived, and disseminated to the public through PDB-Dev. The system currently consists of integrative/hybrid models that follow the specifications defined in the I/H methods dictionary, which is a modular extension of the PDBx/mmCIF dictionary. These include atomistic models, as well as multi-scale models, consisting of different coarse-grained representations
New timsTOF Pro Mass Spectrometer installed at CEITEC.
New timsTOF Pro Mass Spectrometer (Bruker) has been installed at the Proteomics Core Facility, CEITEC MU. The new instrument brings another separation dimension (according collisional cross sections) in qualitative and quantitative characterization of complex protein samples as it is equipped by trapped ion mobility spectrometry (TIMS) module.
Emerging Topics in Biomolecular Magnetic Resonance – A new on-line lecture series
A new on-line lecture series on Emerging Topics in Biomolecular Magnetic Resonance, organized by Loren Andreas, Stefan Glöggler, Christian Griesinger, Mei Hong, Oscar Millet, Art Palmer, and Markus Zweckstetter, starts on Thursday, June 4, 2020.
Cryo-EM reaches yet another milestone – Comments on two recent cryo-EM atomic resolution studies
Two studies published during last two weeks have reported that single particle cryo-EM data can be now resolved to 1.20Å or 1.25Å, respectively. The groups of Sjors H.W. Scheres (LMB-MRC, Cambridge) and Holger Stark (MPI Göttingen) have for the first time shown that atomic resolution is attainable by single particle cryo-EM.
EOSC-Life Training Call Opportunity
The EOSC-Life Training team offers funding to support training activities and provides expertise and guidance to help you organise successful and impactful training through our Training Open Call.