New timsTOF Pro Mass Spectrometer installed at CEITEC.
New timsTOF Pro Mass Spectrometer (Bruker) has been installed at the Proteomics Core Facility, CEITEC MU. The new instrument brings another separation dimension (according collisional cross sections) in qualitative and quantitative characterization of complex protein samples as it is equipped by trapped ion mobility spectrometry (TIMS) module.
Dual TIMS technology and PASEF (Parallel Accumulation Serial Fragmentation) acquisition method provide almost 100% duty cycle and up to 100 Hz acquisition speed without compromising sensitivity and mass resolution. It opens us a way to deeper insights in proteome even in case of low amounts of samples.
The purchase of the instrument was financed by MEYS CR from the Operational Programme: Research, Development and Education, the project „CIISB UP“ (No. CZ.02.1.01/0.0/0.0/18_046/0015974).
Upgrade of the workflow for cryo-electron tomography and microscopy
The CIISB Cryo-electron microscopy core facility at CEITEC Masaryk University is expanding its services in the sample preparation for electron microscopy. The facility has recently acquired high-pressure freezer Leica EM ICE for vitrification of bulky biological specimen (up to 200 mm thickness). In addition, the freeze-substitution unit Leica EM AFS2 for resin embedding of the high-pressure frozen samples and the ultramicrotom Leica EM UC7 with the adapter for cryo-ultramicrotomy were purchased in order to provide the facility users with the complete workflow for preparation of thin section samples for both room-temperature electron microscopy and cryo-electron microscopy.
Emerging Topics in Biomolecular Magnetic Resonance – Nick Cox & Kendra Frederick
The series Emerging topics in Biomolecular Magnetic Resonance will continue on July 9th at 16:00 CEST with the following lecturers and topics:
Nick Cox (Australian National University): Spin state evolution during the biological water splitting reaction
Kendra Frederick (UT Southwestern): In cell structural biology enabled by DNP MAS NMR
Emerging Topics in Biomolecular Magnetic Resonance – 4th edition
The series Emerging topics in Biomolecular Magnetic Resonance will continue on June 25th at 16:00 CEST with the following lecturers and topics:
Melinda J. Duer (University of Cambridge): Understanding extracellular matrix disease states with solid-state NMR?
Claudio Luchinat (University of Florence): NMR for metabolomics: again the ‘second best’ technique?
Emerging Topics in Biomolecular Magnetic Resonance – 3rd edition
The series Emerging topics in Biomolecular Magnetic Resonance will continue on June 18th at 16:00 CEST with the following lecturers and topics:
Tuo Wang (Louisiana State University): Elucidation of carbohydrate structure in plant biomass and fungal pathogens using solid-state NMR and DNP methods
Rina Rosenzweig (Weizmann Institute of Science): Molecular Chaperones in Protein Disaggregation - What we can learn from NMR