New cryo-TEM at CEITEC MU
New mid-range 200 kV transmission electron microscope for cryo-electron microscopy has been installed at Cryo-electron microscopy and tomography core facility CEITEC Masaryk University.
Since October 1, 2018, a new cryo transmission electron microscope Talos Arctica (Thermo Scientific) is fully available to the facility users. The microscope is equipped with the Falcon3 EC direct electron detector and the Ceta camera. The instrument contains the Autoloader device which affords preservation of up to twelve samples inside the microscope. The microscope will be used for automated collection of data for single-particle analysis and structure determination of macromolecular complexes at < 4 A resolution. Moreover, the microscope will allow highly efficient screening and optimization of samples for cryo-electron microscopy. Data for structural characterization of biomacromolecular complexes in situ will be acquired using cryo-electron tomography. In addition, the system will permit applications of electron diffraction tomography for high-resolution structural studies of biomolecules in samples forming micro-crystals.
The purchase of the microscope was financed from the European Regional Development Fund-Project „CIISB4HEALTH“ (No. CZ.02.1.01/0.0/0.0/16_013/0001776).
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