Cryo-EM reaches yet another milestone – Comments on two recent cryo-EM atomic resolution studies
Two studies published during last two weeks have reported that single particle cryo-EM data can be now resolved to 1.20Å or 1.25Å, respectively. The groups of Sjors H.W. Scheres (LMB-MRC, Cambridge) and Holger Stark (MPI Göttingen) have for the first time shown that atomic resolution is attainable by single particle cryo-EM. The cryo-EM maps of apoferritin utilized as a model system in both studies reveal densities of single atoms including the hydrogens. The single particle cryo-EM thus becomes a method capable of reaching atomic resolution. Both studies stress that the use of the electron source with minimal energy dispersion of the emitted electrons is a key for obtaining cryo-EM maps with <1.5Å resolution. This is achieved by utilization of the cold field emission gun (CFEG) or standard Schottky field emission gun and monochromator. Different strategies have been implemented to suppress the optical aberrations which play crucial role at resolutions <1.5Å. The group of H. Stark utilized the newly developed image corrector referenced as BCOR in their Titan Krios microscope to minimize the aberrations directly during data acquisition, whereas the group of S.H.W. Scheres used standard Titan Krios microscope without image corrector and deconvolved the optical aberrations at the level of the data processing within their Relion software. In addition, the study performed at LMB-MRC Cambridge utilizes image filter for filtering out of the inelastically scattered electrons and last generation of the direct electron detector Falcon4. Although the number of particles required to reach the 1.2Å cryo-EM maps differ between the two studies, both papers agree that 1.6-2.0Å resolution maps shall be attainable on the currently standard 300kV cryo-TEMs, which are not equipped with the presented technical advancements.
Webinar series: What can iNEXT-Discovery do for us? Armin Wagner, DIAMOND, Long-wavelength crystallography – Experiments on the edge
The webinar series What can iNEXT-Discovery do for us? will continue on November 30 at 16:00 CEST with the topic Long-wavelength crystallography – Experiments on the edge presented by Armin Wagner, DIAMOND.
[Crystals, IF 2.404] Paper Invitation for Special Issue “Advances in Protein Crystallization and Crystallography “
You are cordially invited to contribute a research article, review, short communication or perspective to a Special Issue of Crystals journal called “Advances in Protein Crystallization and Crystallography “.
Open Research Europe - The European Commission open access publishing platform
European commission is preparing the launch of Open Research Europe, the European Commission scientific publishing service, which will provide Horizon 2020 and Horizon Europe beneficiaries with a venue to publish their results in full compliance with open access policies.
13th Call for proposals for Instruct Centre Training Courses
The Call for Instruct Course for 2021 is now open. This call is for events co-organized with either Instruct-ERIC Centres or Instruct-ERIC research sites, with representatives of the Instruct-ERIC Centre being part of the scientific organizing committee of the course.