Cryo-electron microscopy core facility at CEITEC MUNI has finished installation of a new focused ion beam scanning electron microscope (FIB/SEM). BioHydra (ThermoScientific, Helios Hydra V) is equipped with an immersion SEM optics for high-resolution SEM imaging under room-temperature and cryogenic conditions and FIB working on the principle of inductively-couple plasma with a four gas (Xe,Ar,O,N) source for efficient material ablation. The instrument was proven to deliver 3D reconstruction of whole eukaryotic cells or parts of a tissue preserved at near-native state at resolution better than 30nm. Thus, BioHydra will provide the CIISB user community access to the cryo-volume imaging technology which is rapidly developing over the last few years and extend the capacity of cellular lamellae preparation for in situ structural biology studies by cryo-electron tomography. The installation of BioHydra microscope was made possible through the "Innovation of Czech Infrastructure for Integrative Structural Biology" grant, funded by the OP JAK for Research Infrastructures I.
In CF Diffraction Techniques, we have successfully upgraded the X-ray source of the SAXS instrument. The upgrade of MetalJet C2 to D2+ brought
more than double intensity of the primary beam. The instrument enables rapid standard SAXS measurements with a low volume autosampler, SEC-SAXS
experiments or special applications, now with substantially improved signal to noise ratio.
The Biomolecular Interactions and Crystallography Core Facility of CEITEC MU is very happy to announce the installation of a new cutting-edge instrument that will assist in the characterization of macromolecular complexes. Mass photometer TwoMP (Refeyn) is designed to precisely determine the molecular mass of proteins, nucleic acids, and other macromolecules in a broad range of sizes.