An Instruct/CIISB course took place at the CMS, IBT, Biocev on April 5 and 6, 2018.
This course/workshop allowed participants, with some prior knowledge and experience in macromolecular crystallography, to access the biophysical measurements section and the crystal handling/X-ray diffraction section of the Instruct CZ / CIISB CMS core facility for three practical work sessions.
The course started with a session of seven lectures (preceded by an introduction by Dr Jan Dohnalek, who introduced the Centre of Molecular Structure, the CIISB and Eric-Instruct). The lectures were aimed at providing a comprehensive introduction to the field of fragment and small molecule screening. Of particular interest were the lectures given by three of the invited foreign speakers, who have contributed extensively to the field.
The workshop part of the course (three half days) allowed the participants to acquire practical experience on how to apply fragment-screening techniques to a real case, using endothiapepsin (generous gift of Prof. A. Heine, U. of Marburg). From published work, three fragments were selected for the practical sessions. In addition, an additional three fragments (never studied before) were used during the dry runs for the preparation of the practical sessions. For pre-screening using biophysical methods, the practical work involved both labelled and label-free microscale thermophoresis, and differential scanning fluorimetry. The practical sessions dealing with crystal delivery to a library of fragments allowed crystal handling and transfer using both Intelliplates as well as the Jena Bioscience plates containing a 96-fragment library. The final session (X-ray diffraction) involved data collection using soaked crystals, in-situ data collection as well as the viewing of (difference) Fourier maps showing fragment density.
In all, the course welcomed 19 registered participants working in 11 countries, with invited speakers from Germany and the U.K., together with the local speakers and course tutors. The session of lectures was well attended, with ca. 30 attendees having signed the attendance sheet (the entire audience, including the registered participants and teachers / tutors is estimated at ca. 70).
4th National Day of Large Research Infrastructures
The Ministry of Education, Youth and Sports of the Czech Republic and the Faculty of Mathematics and Physics of Charles University are honoured to invite you to take part in the 4th National Day of Large Research Infrastructures, to be held on 19th November 2019 in the premises of LINDAT/CLARIAH-CZlarge research infrastructure.
Instruct-ERIC International Access Call
We are delighted to announce the opening of the Instruct access call for International users.
Prof Wolfgang Baumeister awarded the 2019 Ivano Bertini Award for outstanding contributions to structural biology
The Ivano Bertini Award is offered by Instruct to recognise a significant achievement in frontier research that utilises an integrative structural biology approach.
CFs succeeded in getting EU support to organize a workshop on the sample quality assessment