An Instruct/CIISB course took place at the CMS, IBT, Biocev on April 5 and 6, 2018.
This course/workshop allowed participants, with some prior knowledge and experience in macromolecular crystallography, to access the biophysical measurements section and the crystal handling/X-ray diffraction section of the Instruct CZ / CIISB CMS core facility for three practical work sessions.
The course started with a session of seven lectures (preceded by an introduction by Dr Jan Dohnalek, who introduced the Centre of Molecular Structure, the CIISB and Eric-Instruct). The lectures were aimed at providing a comprehensive introduction to the field of fragment and small molecule screening. Of particular interest were the lectures given by three of the invited foreign speakers, who have contributed extensively to the field.
The workshop part of the course (three half days) allowed the participants to acquire practical experience on how to apply fragment-screening techniques to a real case, using endothiapepsin (generous gift of Prof. A. Heine, U. of Marburg). From published work, three fragments were selected for the practical sessions. In addition, an additional three fragments (never studied before) were used during the dry runs for the preparation of the practical sessions. For pre-screening using biophysical methods, the practical work involved both labelled and label-free microscale thermophoresis, and differential scanning fluorimetry. The practical sessions dealing with crystal delivery to a library of fragments allowed crystal handling and transfer using both Intelliplates as well as the Jena Bioscience plates containing a 96-fragment library. The final session (X-ray diffraction) involved data collection using soaked crystals, in-situ data collection as well as the viewing of (difference) Fourier maps showing fragment density.
In all, the course welcomed 19 registered participants working in 11 countries, with invited speakers from Germany and the U.K., together with the local speakers and course tutors. The session of lectures was well attended, with ca. 30 attendees having signed the attendance sheet (the entire audience, including the registered participants and teachers / tutors is estimated at ca. 70).
ARIA Workshop and User Group meeting
Instruct-ERIC is hosting an ARIA workshop and User Group meeting on 25th February in Amsterdam.
CIISB UP project approved for financing during 2020-2022
The project CIISB UP submitted to the OP VVV Call 02-18-046 Research Infrastructures II has been positively evaluated and will receive funding to reinvest into the existing equipment and to purchase new instrumentation in the amount 22 mil. EUR.
Magnetic Resonance - An Interactive Open Access Publication of the Groupement AMPERE
The AMPERE Society has decided to launch a new publication called “Magnetic Resonance, An Interactive Open Access Publication of the Groupement AMPERE” for articles on Nuclear and Electron Magnetic Resonance and Imaging.
Advanced SAXSanalysis trainingWe would like to invite you to an Advanced SAXSanalysis training.The training is designed to help you with processing data collected with small angle X-ray scattering (SAXS) instrument in the Centre of Molecular Structure in the Institute of Biotechnology of the Czech Academy of Sciences in BIOCEV. The tutorials will be by application specialist Anton Paar (instrument manufacturer).