switchSENSE technology presentation

  • 13 April 2018
    11:00 AM
  • Room U1.038, Biotechnologický ústav AV ČR, v. v. i., Průmyslová 595, 252 50 Vestec, Česká republika

switchSENSE® is an automated biosensor chip technology that employs electrically actuated DNA nanolevers for the real-time measurement of binding kinetics (kON, kOFF) and affinities (KD). Interactions between proteins, DNA/RNA, and small molecules can be detected with femto-molar sensitivity. 

At the same time, protein diameters (DH) are analyzed with 0.1 nm accuracy and conformational changes as well as melting transitions (TM) can be measured using minimal amounts of sample. 

The principles and applicability of three complementary measurement modalities of switchSENSE® will be introduced in this talk: Fluorescence Proximity Sensing, Molecular Ruler Measurements, and Switching Dynamics Measurements. 

We will discuss workflows and unique possibilities for monitoring protein size and assembly of biomolecular complexes in solution by assessing the hydrodynamic radius.

Presented topics:

  • Conformational Changes in Proteins 
  • DNA/RNA binding Proteins & Enzymes

 Date: April 13th, 2018

 Time: 11:00 - 01:30 pm

For more information please contact Dr. Tatsiana Charnavets: tatsiana.charnavets@ibt.cas.cz.

Seminar Registration Deadline is April 11th, 2018. To register for this event please write a message to events@dynamic-biosensors.com stating your full name and institution.

Type of event: Presentation
Core facility: Biophysical techniques
Partner: BIOCEV

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