Protein Production Hands-On Workshop

• Dates: November 3 – 5, 2026
• Venue: BIOCEV, Vestec Registration
• Cost: Free of charge (accommodation not included)

Workshop Overview

This three-day course offers both foundational and practical training in recombinant protein production. Participants will gain hands-on experience across the entire workflow – from cell transformation, recombinant protein expression and purification to final sample concentration and purity validation.

Format

Primarily hands-on tutorials, complemented by short lectures
Participants will work in pairs to express and purify recombinant protein
Lectures are open to external attendees upon prior consultation (due to seminar room capacity)
Workshop capacity is limited; registration is required

Tutorials and lectures will be led by technical scientists from the CMS Protein Production Core Facility:
Miroslava Alblova, Tereza Kolarcikova, Veronika Klapstova, Agnieszka Szmitkowska, Lenka Vidrnova, Pavlina Jaklova

Preliminary Programme

Tuesday, November 3 – Protein Expression in E. coli (9:00 – 17:00)
09:00 – 09:20: Introduction (lecture)
10:00 – 10:30: Preparation for protein expression in 1 L E. coli culture (practical)
10:30 – 11:00: Cloning using restriction enzymes and RF cloning (lecture)
11:00 – 11:30: Overview of prokaryotic and eukaryotic expression systems we use (lecture)
12:00 – 14:00: OD measurements and induction of protein expression (practical)
14:00 – 16:00: E. coli transformation and group discussion
16:00 – 17:00: Chromatography techniques (lecture)

Wednesday, November 4 – Cell Harvesting & Initial Purification (9:00 – 16:00)
09:00 – 10:00: Cell harvesting (practical)
10:00 – 12:00: Sonication and centrifugation (practical)
13:00 – 16:00: Affinity chromatography, SDS-PAGE, dialysis (practical)
14:00 – 14:15: Media preparation (lecture)

Thursday, November 5 – Final Purification & Troubleshooting (9:00 – 15:00)
09:00 – 15:00: Protein concentration, Size Exclusion Chromatography, SDS-PAGE (practical)
09:00 – 15:00: Open discussions and troubleshooting