Lecture of Dr. Victor A. Lorenz-Fonfria from the Institute of Molecular Science in Spain
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3 July 2019
3:00 PM
Dear colleagues,
we would like to invite you to the lecture of Dr. Victor A. Lorenz-Fonfria from the Institute of Molecular Science (ICMol), Spain "Time-resolved FT-IR difference spectroscopy of proteins: from principles to applications".
- Date: 3rd of July 2019
- Time: 15:00
- Location: U1.037, BIOCEV
Abstract of lecture:
Time-resolved FT-IR difference spectroscopy of proteins: from principles to applications
Victor A. Lorenz-Fonfria
Institute of Molecular Science (ICMol), Universitat de València, 46980 Paterna, Spain
FTIR spectroscopy is sensitive to molecular vibrations. But when applied to proteins, FTIR spectroscopy suffers from a strong background absorbance from the solvent and from band crowding due to the many overlapping vibrations present in a protein. These two problems are reduced by means of FTIR difference spectroscopy, which examines the differences in absorbance before and after a physiologically relevant perturbation that alters the state of a protein (e.g., light excitation for light- sensitive proteins). After the protein is perturbed, for instance with a short laser pulse, FTIR spectra can be recorded as a function of time. The step-scan technique allows to obtain FTIR spectra from 10 ns to 100 ms, while the rapid-scan technique allow to obtain FTIR spectra from 10 ms until tens of seconds or even tens of minutes. When applied to proteins, time-resolved FTIR difference spectroscopy can provide information about changes in the protonation state of amino acid residues, chromophores/cofactors, internal water molecules and buffer molecules, as well as their time sequence. These changes in protonation state can then be used to identify proton transfer reactions and, in the case of proton- pumps, to devise a proton-pumping mechanism.
Here, first I will introduce the idea behind FTIR difference spectroscopy of proteins. Then, I will explain the time-resolved rapid-scan and step-scan techniques. After giving the foundations, I will explain how time-resolved FTIR difference spectroscopy was applied to resolve the proton transfer reactions in the light-driven proton pump bacteriorhodopsin 1–3, and the light-gated ion channel channelrhodopsin-2 4–6.
- Lórenz-Fonfría, V. a & Kandori, H. Spectroscopic and kinetic evidence on how bacteriorhodopsin accomplishes vectorial proton transport under functional conditions. J. Am. Chem. Soc. 131, 5891–901 (2009).
- Lórenz-Fonfría, V. a., Kandori, H. & Padrós, E. Probing specific molecular processes and intermediates by time-resolved Fourier transform infrared spectroscopy: application to the bacteriorhodopsin photocycle. J. Phys. Chem. B 115, 7972– 85 (2011).
- Lorenz-Fonfria, V. A., Saita, M., Lazarova, T., Schlesinger, R. & Heberle, J. pH-sensitive vibrational probe reveals a cytoplasmic protonated cluster in bacteriorhodopsin. Proc. Natl. Acad. Sci. 201707993 (2017). doi:10.1073/pnas.1707993114
- Lórenz-Fonfría, V. a et al. Transient protonation changes in channelrhodopsin-2 and their relevance to channel gating.Proc. Natl. Acad. Sci. U. S. A. 110, E1273-81 (2013).
- Lórenz-Fonfría, V. a et al. Pre-gating conformational changes in the ChETA variant of channelrhodopsin-2 monitored by nanosecond IR spectroscopy. J. Am. Chem. Soc. 137, 1850–61 (2015).
- Lórenz-Fonfría, V. A. et al. Temporal evolution of helix hydration in a light-gated ion channel correlates with ion conductance. Proc. Natl. Acad. Sci. 201511462 (2015). doi:10.1073/pnas.1511462112
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