Upgrades of the differential scanning fluorimetry instruments Prometheus NT.48 at BIOCEV and CEITEC
Recently, both instruments Prometheus NT.48 at BIOCEV and CEITEC, permitting precise measurements of the intrinsic fluorescence of proteins, thereby characterizing their thermal unfolding, have been upgraded to include back-reflection optics, which provides insight into the aggregation processes. The basis for such detection is the aggregation-induced light scattering by particles resulting in loss of light. The combination of thermal stability detection and determination of aggregation onset temperatures allows for the most rapid, precise, and information rich analysis in formulation screenings or protein engineering projects. The upgraded instruments now can provide comprehensive results for any type of proteins, and are especially suitable for the prediction of proteins stability, for the screening of buffer influence, and for the testing of storage conditions. Part of the upgrade was also increase in the maximum possible temperature from original 95°C to 110°C . This enhancement allows to characterize proteins with higher melting temperature. Both upgrades were financed from the European Regional Development Fund-Project „CIISB4HEALTH“ (No. CZ.02.1.01/0.0/0.0/16_013/0001776).
Nadrian C. Seeman: DNA, not Merely the Secret of Life
We build branched DNA species that can be joined using Watson-Crick base pairing to produce N-connected objects and lattices. We have used ligation to construct DNA topological targets, such as knots, polyhedral catenanes, Borromean rings and a Solomon's knot.
An Instruct/CIISB course took place at the CMS, IBT, Biocev on April 5 and 6, 2018.
This course/workshop allowed participants, with some prior knowledge and experience in macromolecular crystallography, to access the biophysical measurements section and the crystal handling/X-ray diffraction section of the Instruct CZ / CIISB CMS core facility for three practical work sessions.
XV. Discussions in Structural Molecular Biology and the 2nd User Meeting of the Czech Infrastructure for Integrative Structural Biology
In March, 2018, the Czech and Slovak community of structural biologists met at the "XV. Discussions in Structural Molecular Biology".
1st CEITEC Symposium on Recent Advances in Cryo-Electron Microscopy
Cryo-electron Microscopy and Tomography CEITEC Masaryk University has organized a one day symposium on recent developments in the field of cryo-electron microscopy on 18thApril 2018.