Titan Krios transmission electron microscope at CEITEC equipped with phase plate and last generation direct electron detectors

12 February 2018

Transmission electron microscope Titan Krios  at Cryo-electron microscopy and tomography core facility CEITEC MU has been recently upgraded with phase plate, and two last generation direct electron detectors (FEI Falcon3EC, Gatan K2). Phase plate significantly increases contrast in cryo-electron microscopy data and allows to collect data at in-focus conditions. Moreover, increased contrast extends applicability of cryo-electron microscopy to high-resolution structural characterization of proteins and protein complexes which are around 100kDa in size. Both newly acquired direct electron detectors operate in electron counting mode, which provides superior image quality. Besides, the Gatan K2 detector position is behind the post-column energy filter and allows zero-loss imaging. Such application is primarily beneficial for imaging of thicker samples such as vitrified cells or focused ion beam micromachined cellular lamellas using cryo-electron tomography  for the purpose of structural characterization of biomacromolecular complexes in situ. All upgrades were financed from the European Regional Development Fund-Project „CIISB4HEALTH“ (No. CZ.02.1.01/0.0/0.0/16_013/0001776).

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