Practical NMR Spectroscopy of Biomolecules

The students will get hands-on experience with using NMR spectrometers with an accent on techniques for spectroscopy of proteins and nucleic acids. All important exprimental issues from sample preparation and spectrometer setup and calibration trough data aquisition and processing up to spectra evaluation will be discussed as well as practically performed in the laboratory. At the end of the course, the students will be able to use independently the sofisticated NMR instrumentation for routine measurements of spektra of proteins and nucleic acids, as well as most other common samples.

Prerequisites: Students should have basic knowledge of NMR theory

Registration: Masaryk University students, register for the course C7995, Others, contact Radovan Fiala at radovan.fiala@ ceitec.muni.cz

Dates and times: September – October 2019, exact dates and times will be decided based on the availability of teachers and spectrometers and on student preferences

Type of event: Training
Core facility: Josef Dadok National NMR Centre
Partner: CEITEC

Syllabus

• NMR sample, preparation and handling
• Spectrometer setup
• Spectrometer calibrations
• 1D proton spectroscopy and water suppression techniques
• 1D NMR spectroscopy of nuclei other than H-1
• Homonuclear 2D experiments
• Heteronuclear experiments for natural abundance and isotopically labeled samples
• Data processing, apodization functions, linear prediction, non-uniform sampling
• Analysis of protein NMR spectra
• Analysis of NMR spectra of nucleic acids

Literature

• NMR of macromolecules : a practical approach. Edited by Gordon C.K. Roberts. Oxford: Oxford University Press, 1993. 399 s. ISBN 0-19-963224-3. info
• BERGER, Stefan a Siegmar BRAUN. 200 and more NMR experiments :a practical course. Weinheim: Wiley-VCH, 2004. xv, 838. ISBN 3527310673.
• CAVANAGH, John. Protein NMR spectroscopy :principles and practice. 2nd ed. Amsterdam: Elsevier, 2007. xxv, 885. ISBN 9780121644918. infoinfo