Biomolecular Interactions and Crystallization

The core facility provides services leading to structure characterization of biomolecules and to study (bio)molecular interactions in a real time using mainly biosensor and calorimetry-based methods. It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, CD spectroscopy, analytical ultracentrifugation).

Key Equipment

  • Crystallization robotics (Phoenix, Mosquito LCP, DragonFly)
  • Automated Plate Storage and InspectionMinstrel HT- UV + Gallery HT Hotels (4°C and 20°C)
  • Temperature optimizer for crystallization TG40 for 5 different temperatures
  • 96-well UVP screening plates and 24-well optimization plates 
  • SPR BiaCore T200
  • Calorimeters Auto-iTC200, VP-ITC, VP-DSC  
  • Microscale thermophoresis Monolith NT.115
  • Analytical ultracentrifuge ProteomLab XLI
  • CD spectrometer Jasco 850 equipped with fluorescence detector
  • DLS Dynapro Plate Reader, DelsaMax
  • Differential scanning fluorimeter Prometheus NT.48 with aggregation detection optics

Expertise

  • High throughput screening and optimization of conditions for an ideal growth of perfect protein crystal(s)
  • Studies of physical properties of the molecules
  • Characterization of (bio)molecular interactions including thermodynamics and/or kinetics parameters

Open-access

    • Crystallization of biomolecules and their complexes
    •  Basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry)
    • Studying of thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, CD spectroscopy, analytical ultracentrifugation)

Core Facility contact

Michaela Wimmerová - michaela.wimmerova@ceitec.muni.cz

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More information aboutCore Facility Biomolecular Interaction and Crystalization and related services you can find in document Equipement and services of CIISB.


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