Biomolecular Interactions and Crystallization

The core facility provides services leading to structure characterization of biomolecules and to study (bio)molecular interactions in a real time using mainly biosensor and calorimetry-based methods. It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, CD spectroscopy, analytical ultracentrifugation).

Key Equipment

    • NMR spectrometer for high-resolution spectroscopy in liquids (600 MHz, 700 MHz, 850 MHz and 950 MHz)
    • Crystallization robot Mosquito and Tecan Evo 150
    • Automated Desktop UV Minstrel + Gallery Hotel 160 for inspection of screening plates
    • Leica microscope with polarizing filter • Temperature optimizer for crystallization TG40 for 5 different temperatures
    • 96-well UVP screening plates and 24-well optimization plates
    • SPR BiaCore 3000
    • Calorimeters AutoITC200, VP-ITC, VP-DSC
    • Analytical ultracentrifuge ProteomLab XLI
    • CD spectrometer Jasco 850 equipped with fluorescence detector
    • DLS Dynapro Plate Reader, DelsaMax

Expertise

    • High throughput screening and optimization of conditions for an ideal growth of perfect protein crystal(s)
    • Studies of physical properties of the molecules
    • Characterization of (bio)molecular interactions in real time using mainly biosensor and calorimetry-based methods

Open-access

    • Crystallization of biomolecules and their complexes
    •  Basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry)
    • Studying of thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, CD spectroscopy, analytical ultracentrifugation)

Core Facility contact

Michaela Wimmerová - michaela.wimmerova@ceitec.muni.cz

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More information aboutCore Facility Biomolecular Interaction and Crystalization and related services you can find in document Equipement and services of CIISB.


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