The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec near Prague, Central Bohemia) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes at different resolution levels, ranging from atomic to cellular.
CIISB, equipped with the cutting-edge technologies, offers access to the equipment and expertise in the following fields: high-field NMR spectroscopy, high throughput crystallization of biological macromolecules, X-ray diffraction techniques, Bio-SAXS measurements, AFM imaging, characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, analytical ultracentrifugation, and surface plasmon resonance, high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells, high-end mass spectrometry characterization of biomacromolecules, determination of protein post-translational modifications, MS-based peptide mapping/sequencing, high throughput sequencing and proteomic services, chemical cross-linking and H/D exchange. CIISB infrastructure provides high quality open access services in provision of methods and techniques on a level of national and international importance.
CIISB reinforces the national and international position of Integrative Structural Biology in the Czech Republic by providing the scientific community with competitive tools and aims to foster the development of innovative technologies by companies and their effective uptake by academic and industrial researchers in the Czech Republic and Europe. Progress of cellular structural biology is driven by innovations at the boundary between technologies stimulating research that integrates an understanding of biological structures with cellular function. Cutting-edge instrumentation installed at CIISB offers opportunity to expand deeply our knowledge of complex cellular systems.
Czech Infrastructure for Integrative Structural Biology (CIISB) is supported by the MŠMT project projects LM2015043, 2016-2019 and CIISB4HEALTH CZ.02.1.01/0.0/0.0/16_013/0001776.
Project iNEXT (H2020 project #653706, 2015-2019) - the first research infrastructure project that combines access to different structural biology technologies (SAXS, X-ray crystallography, NMR, EM, biophysical characterization) - supports access to CIISB high-field NMR and cryo-EM Core Facilities.
Project INSTRUCT-ULTRA (H2020 project #731005, 2016-2020) - accelerating the expanded implementation of INSTRUCT – supports development of integrated structural biology approaches combining solution state NMR spectroscopy and cryo-electron microscopy (EM).
The Czech Republic is a member of INSTRUCT ERIC , a European Infrastructure Research Consortium (part of the ESFRI Roadmap for Europe since 2006 and ESFRI Landmark since 2016). CIISB, together with the Czech Society of Structural Biology (CSSB), represents the national structural biology resources and community in INSTRUCT ERIC. This arrangement ensures tight connection to the European community of structural biologists, efficient transfer of information and know-how and allows for adequate representation of national interest at the European and international level. Activities of CIISB in the ESFRI project INSTRUCT ERIC consist in providing the functionality of a national node and reference centre in the offered techniques, including biomolecular crystallography, NMR techniques, cutting edge electron microscopy, structural mass spectrometry, biophysical and interaction-probing techniques and complementary computational approaches.
CIISB, as a part of CEITEC and BIOCEV projects, has direct links also to other European ESFRI projects. Both CEITEC and BIOCEV participate in the ELIXIR, European infrastructure for biological information, supporting life science research and its translation to medicine, agriculture, bioindustries, and society, representing essential parts of the Czech ELIXIR node. CEITEC and BIOCEV also actively contribute to EUROBIOIMAGING, a project to build a distributed imaging infrastructure across Europe that will provide open access to innovative biological and medical imaging technologies for European researchers. Through their home institutions, Masaryk University and the Institute of Molecular Genetics, Institute of Biotechnology and Charles University, CEITEC and BIOCEV have a direct connection to another ESFRI project EU-OPENSCREEN, the European Infrastructure of Open Screening Platforms for Chemical Biology aiming to deliver new biologically active compounds for all fields of the Life Sciences. CIISB is linked also to the ESFRI project INFRAFRONTIER, which aims to build a world-class research infrastructure that provides the biomedical research community with the tools needed to unravel the role of gene function in human disease. The Czech Centre for Phenogenomics at the Institute of Molecular Genetics - BIOCEV is a founding member of this project. CIISB is connected also to EATRIS, European Infrastructure on Translational Medicine transforming laboratory research outcomes into new ways to diagnose and treat patients to improve the health of people worldwide, through participation of the CEITEC Molecular Medicine programme. Masaryk University is also representing a national hub of ECRIN, a European network dedicated to improving the health of patients and citizens across the world through clinical research.